Composite

Part:BBa_K3052021:Design

Designed by: Ma WenHe   Group: iGEM19_XJTU-CHINA   (2019-10-13)


light controlling circuit - LGF


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 5456
    Illegal PstI site found at 5975
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 6963
    Illegal NheI site found at 6986
    Illegal PstI site found at 5456
    Illegal PstI site found at 5975
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2298
    Illegal BglII site found at 5287
    Illegal BglII site found at 6249
    Illegal BglII site found at 6297
    Illegal BglII site found at 8523
    Illegal XhoI site found at 405
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 5456
    Illegal PstI site found at 5975
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 5456
    Illegal PstI site found at 5975
    Illegal NgoMIV site found at 5608
    Illegal NgoMIV site found at 8076
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The expression level of the two phases of this switch may be different.


Source

This part is acquired from chemical synthesis.

References